The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture

Hecht, V., Vielle-Calzada, J. P., Hartog, M. V., Schmidt, E. D. L., Boutilier, K., Grossniklaus, U., de Vries, S. C. (November 2001) The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiology, 127 (3). pp. 803-816. ISSN 0032-0889

URL: http://www.ncbi.nlm.nih.gov/pubmed/11706164
DOI: ​10.​1104/​pp.​010324

Abstract

We report here the isolation of the Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1 (AtSERK1) gene and we demonstrate its role during establishment of somatic embryogenesis in culture. The AtSERK1 gene is highly expressed during embryogenic cell formation in culture and during early embryogenesis. The AtSERK1 gene is first expressed in planta during megasporogenesis in the nucleus of developing ovules, in the functional megaspore, and in all cells of the embryo sac up to fertilization. After fertilization, AtSERK1 expression is seen in all cells of the developing embryo until the heart stage. After this stage, AtSERK1 expression is no longer detectable in the embryo or in any part of the developing seed. Low expression is detected in adult vascular tissue. Ectopic expression of the full-length AtSERK1 cDNA under the control of the cauliflower mosaic virus 35S promoter did not result in any altered plant phenotype. However, seedlings that overexpressed the AtSERK1 mRNA exhibited a 3- to 4-fold increase in efficiency for initiation of somatic embryogenesis. Thus, an increased AtSERK1 level is sufficient to confer embryogenic competence in culture.

Item Type: Paper
Uncontrolled Keywords: LEUCINE-RICH REPEAT PROTEIN-KINASE DEVELOPMENTAL PATHWAY MERISTEM DEVELOPMENT PLANT EMBRYOGENESIS SIGNAL-TRANSDUCTION THALIANA CLAVATA1 CELLS APOMIXIS
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > developmental stage > embryo
organs, tissues, organelles, cell types and functions > tissues types and functions > embryogenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Grossniklaus lab
Depositing User: Matt Covey
Date: November 2001
Date Deposited: 22 Jan 2014 19:43
Last Modified: 22 Jan 2014 19:43
PMCID: PMC129253
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29243

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