Expression of c-Fos, Fos B, Jun B, and Zif268 transcription factor proteins in rat barrel cortex following apomorphine-evoked whisking behavior

Filipkowski, R. K., Rydz, M., Kaczmarek, L. (2001) Expression of c-Fos, Fos B, Jun B, and Zif268 transcription factor proteins in rat barrel cortex following apomorphine-evoked whisking behavior. Neuroscience, 106 (4). pp. 679-688. ISSN 0306-4522

URL: http://www.ncbi.nlm.nih.gov/pubmed/11682155
DOI: 10.1016/S0306-4522(01)00310-4

Abstract

Apomorphine-evoked expression of transcription factor proteins: c-Fos, Fos B, Jun B, and Zif268 (also named Krox-24, NGFI-A, Egr-1), was investigated in rat somatosensory (barrel) cortex. The effect of the X-methyl-D-aspartate receptor antagonist MK-801 on their expression was also analyzed. Apomorphine is a dopamine receptor agonist, eliciting motor activity, including enhanced whisking leading to the activation of vibrissae representation in the barrel cortex. Rats had their whiskers clipped on one side of the snout. The Zif268 levels were markedly reduced by this procedure alone. In contrast, apomorphine (5.0 mg/kg) evoked marked c-Fos elevation, less pronounced changes in Jun B and Zif268 and no change in Fos B. The greatest apomorphine-evoked c-Fos accumulation was observed in layers IV and V/VI of non-deprived barrel cortex and was not significantly influenced by MK-801 injection at 0.1 mg/kg. A higher dose of MK-801 (1.0 mg/kg) produced abnormalities in locomotor behavior and diminished c-Fos levels on the non-deprived side to the ones observed in the sensory stimulus-deprived cortex. We conclude that the response of the somatosensory cortex is selective with respect to both the gene activated and its cortical layer localization. Furthermore, sensory stimulation provides a major but not the only component to apomorphine-evoked barrel cortex gene activation.

Item Type: Paper
Uncontrolled Keywords: vibrissae NMDA receptor immediate early genes AP-1 MK-801 IMMEDIATE-EARLY GENES RECEPTOR ANTAGONIST MK-801 DNA-BINDING ACTIVITY CEREBRAL-CORTEX NERVOUS-SYSTEM VISUAL-CORTEX MESSENGER-RNA LONG-TERM DOPAMINERGIC STIMULATION DIFFERENTIAL REGULATION
Subjects: organism description > animal behavior
organs, tissues, organelles, cell types and functions > tissues types and functions > barrel cortex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Yin lab
Depositing User: Matt Covey
Date: 2001
Date Deposited: 22 Jan 2014 17:03
Last Modified: 22 Jan 2014 17:03
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29232

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