DNA recognition by the herpes simplex virus transactivator VP16: a novel DNA-Binding structure

Babb, R., Huang, C. C., Aufiero, D. J., Herr, W. (July 2001) DNA recognition by the herpes simplex virus transactivator VP16: a novel DNA-Binding structure. Molecular and Cellular Biology, 21 (14). pp. 4700-4712. ISSN 0270-7306

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11416146
DOI: 10.1128/MCB.21.14.4700-4712.2001

Abstract

Upon infection, the herpes simplex virus (HSV) transcriptional activator VP16 directs the formation of a multiprotein-DNA complex-the VP16-induced complex-with two cellular proteins, the host cell factor HCF-1 and the POU domain transcription factor Oct-1, on TAATGARAT-containing sequences found in the promoters of HSV immediate-early genes. HSV VP16 contains carboxy-terminal sequences important for transcriptional activation and a central conserved core that is important for VP16-induced complex assembly. On its own, VP16 displays little, if any, sequence-specific DNA-binding activity. We show here that, within the VP16-induced complex, however, the VP16 core has an important role in DNA binding. Mutation of basic residues on the surface of the VP16 core reveals a novel DNA-binding surface with essential residues which are conserved among VP16 orthologs. These results illuminate how, through association with DNA, VP16 is able to interpret cis-regulatory signals in the DNA to direct the assembly of a multiprotein-DNA transcriptional regulatory complex.

Item Type: Paper
Uncontrolled Keywords: OCT-1 POU DOMAIN EARLY GENE-EXPRESSION RNA-POLYMERASE-II REGULATORY PROTEIN CRYSTAL-STRUCTURE HOMEO DOMAIN TRANSCRIPTIONAL REGULATION CONFORMATIONAL ALTERATION VP16-INDUCED COMPLEX TAATGARAT MOTIFS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > DNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organism description > virus > herpes simplex virus
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: July 2001
Date Deposited: 22 Jan 2014 20:46
Last Modified: 22 Jan 2014 20:46
PMCID: PMC8714
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29218

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