Molecular cloning and characterization of iojap (ij), a pattern striping gene of maize

Han, C. D., Coe, E. H., Martienssen, R. A. (1992) Molecular cloning and characterization of iojap (ij), a pattern striping gene of maize. EMBO J, 11 (11). pp. 4037-4046. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/1382980

Abstract

Iojap (ij) is a recessive striped mutant of maize affecting the development of plastids in a local and position-dependent manner on the leaves. The ij-affected plastids are transmitted to some of the progeny even when the function of the nuclear gene is restored. Developmental defects during embryogenesis and leaf proliferation are other phenotypic characteristics of ij. The extent of striping and the degree of developmental arrest in ij depend upon genetic background. To understand the diverse and unique phenotypic expression of ij, a transposon tagging experiment has been conducted using Robertson's Mutator (Mu). A new ij mutant was obtained from crosses of the reference allele of (ij-ref) to Mu lines. Subsequent genetic and molecular studies showed that the mutant carried a new ij allele (ij-mum1) from the Mu lines and contained a Mu1 element that cosegregated with the iojap phenotype. A 6.0 kb EcoRI genomic DNA fragment containing the Mu1 element was cloned. ij-ref is unstable, and revertants (Ij-Rev) have been obtained. Using the flanking DNA from the genomic clone as a probe, DNA polymorphisms were detected between ij-ref and these revertants. Further, transcripts were restored to the normal level in Ij-Rev seedlings. Comparison of genomic DNA clones from ij-ref, ij-mum1 and Ij indicated that the ij-ref allele contained 1.5 kb of additional DNA related to a transposable element, Ds. Germinal and somatic revertant alleles were derived by excision of this 1.5 kb element from ij-ref. The structure of the Ij gene and the DNA sequence of its transcribed region were determined. The Ij gene encodes a 24.8 kDa protein that showed no significant sequence similarity with proteins listed in databases.

Item Type: Paper
Uncontrolled Keywords: Alleles Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular DNA DNA Transposable Elements Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic RNA Restriction Mapping Zea mays
Subjects: organism description > plant > maize
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > plant
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: 1992
Date Deposited: 07 Jan 2014 14:51
Last Modified: 07 Jan 2014 14:51
PMCID: PMC556914
URI: http://repository.cshl.edu/id/eprint/29201

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