Amplification of calcium-induced gene transcription by nitric oxide in neuronal cells

Peunova, N., Enikolopov, G. (1993) Amplification of calcium-induced gene transcription by nitric oxide in neuronal cells. Nature, 364 (6436). pp. 450-453. ISSN 0028-0836

URL: http://www.ncbi.nlm.nih.gov/pubmed/8392663
DOI: 10.1038/364450a0

Abstract

NITRIC oxide (NO) is a short-lived, highly reactive gas, which has been identified as a mediator in vasodilation, an active agent in macrophage cytotoxicity and neurotoxicity, and a neurotransmitter in the central and peripheral nervous systems1-5. Production of NO by neurons is critical for facilitated synaptic transmission in models of synaptic plasticity such as long-term potentiation and long-term depression, suggesting a role for NO as a retrograde messenger that could complete a hypothetical feed-back loop by strengthening the connection between postsynaptic and presynaptic cells6-10. We report here that although alone NO has no evident effect on transcription, it can act as an amplifier of calcium signals in neuronal cells. NO and Ca2+ action have to coincide in time for amplification to occur. Experiments with a series of simplified reporter genes in combination with specific recombinant protein kinase inhibitors suggest that induction of gene activity following NO-amplified calcium action involves protein kinase A-dependent activation of the transcription factor CREB.

Item Type: Paper
Uncontrolled Keywords: LONG-TERM POTENTIATION CYCLIC-AMP C-FOS NERVOUS-SYSTEM FACTOR CREB BINDING-PROTEIN PHOSPHORYLATION MESSENGER SEQUENCE KINASES
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > calcium channel
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > small molecules > nitric oxide
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Matt Covey
Date Deposited: 20 Dec 2013 16:07
Last Modified: 20 Dec 2013 16:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29142

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