Specific pattern of p53 phosphorylation during nitric oxide-induced cell cycle arrest

Nakaya, N., Lowe, S. W., Taya, Y., Chenchik, A., Enikolopov, G. (December 2000) Specific pattern of p53 phosphorylation during nitric oxide-induced cell cycle arrest. Oncogene, 19 (54). pp. 6369-6375. ISSN 0950-9232

URL: http://www.ncbi.nlm.nih.gov/pubmed/11175352
DOI: 10.1038/sj.onc.1204100

Abstract

Nitric oxide (NO) is an efficient inhibitor of cell proliferation. Here we show that part of the antiproliferative activity of NO in fibroblasts is mediated through p53 signaling pathway. Cells from p53-/- knockout mice are compromised in their ability to stop dividing in the presence of NO, NO strongly induces expression of genes which are transcriptional targets of p53, and p53 is necessary for some, but not all, of the transcription activation effects of NO, Furthermore, NO strongly increases the cellular level of p53 protein. Since phosphorylation of particular residues of the p53 molecule has been correlated with its functional activity, we determined the phosphorylation pattern of p53 molecule after exposure to NO and compared it with the phosphorylation patterns that develop upon treatment with gamma-irradiation, UV light, and adriamycin. We found that NO induces a specific signature pattern of p53 phosphorylation, distinct from the patterns evoked by other inducers. This study suggests that NO activates specific signaling pathways that may partially overlap, but that do not coincide, with signaling pathways activated by other known inducers of p53 activity.

Item Type: Paper
Uncontrolled Keywords: nitric oxide p53 proliferation cell cycle arrest phosphorylation DNA-DAMAGE RIBONUCLEOTIDE REDUCTASE DROSOPHILA DEVELOPMENT GROWTH APOPTOSIS DIFFERENTIATION PROLIFERATION ACTIVATION INHIBITION RADIATION
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > small molecules > nitric oxide
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
CSHL labs > Lowe lab
Depositing User: Matt Covey
Date: December 2000
Date Deposited: 20 Dec 2013 15:40
Last Modified: 20 Dec 2013 15:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29134

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