A novel regulator of p21-activated kinases

Bagrodia, S., Taylor, S. J., Jordon, K. A., Van Aelst, L., Cerione, R. A. (September 1998) A novel regulator of p21-activated kinases. Journal of Biological Chemistry, 273 (37). pp. 23633-23636. ISSN 0021-9258

URL: http://www.ncbi.nlm.nih.gov/pubmed/9726964
DOI: 10.1074/jbc.273.37.23633

Abstract

Proteins of the pal-activated kinase (Pak) family have been implicated in the regulation of gene expression, cytoskeletal architecture, and apoptosis, Although the ability of Cdc42 and Pac GTPases to activate Pak is well established, relatively little else is known about Pak regulation or the identity of Pak cellular targets. Here we report the identification of two closely related Pak3-binding proteins, possibly arising from alternative splicing, designated p50 and p85(Cool-1) (cloned out of library), Both isoforms of Cool contain a Src homology 3 domain that directly mediates interaction with Pak3 and tandem Dbl homology and pleckstrin homology domains. Despite the presence of the Dbl homology-pleckstrin homology motif, a characteristic of Rho family activators, activation of Cdc42 or Rac by Cool is not detectable. Instead binding of p50(Cool-1), but not p85(Cool-1), to Pak3 represses its activation by upstream activators such as the Dbl oncoprotein, indicating a novel mechanism of regulation of Pak signaling.

Item Type: Paper
Uncontrolled Keywords: ACTIVATED PROTEIN-KINASE ACTIN STRESS FIBERS RAS TRANSFORMATION DEPENDENT ACTIVATION FOCAL COMPLEXES CDC42 GTPASES RHO RAC1 MEMBRANE FAMILY
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p21
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Van Aelst lab
Depositing User: Matt Covey
Date: September 1998
Date Deposited: 18 Dec 2013 21:31
Last Modified: 18 Dec 2013 21:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29111

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