Rho GTPases regulate distinct aspects of dendritic arbor growth in Xenopus central neurons in vivo

Li, Z., Van Aelst, L., Cline, H. T. (2000) Rho GTPases regulate distinct aspects of dendritic arbor growth in Xenopus central neurons in vivo. Nature Neuroscience, 3 (3). pp. 217-225. ISSN 1097-6256

URL: http://www.ncbi.nlm.nih.gov/pubmed/10700252
DOI: 10.1038/72920

Abstract

The development and structural plasticity of dendritic arbors are governed by several factors, including synaptic activity, neurotrophins and other growth-regulating molecules. The signal transduction pathways leading to dendritic structural changes are unknown, but likely include cytoskeleton regulatory components. To test whether GTPases regulate dendritic arbor development, we collected time-lapse images of single optic tectal neurons in albino Xenopus tadpoles expressing dominant negative or constitutively active forms of Rac, Cdc42 or RhoA. Analysis of images collected at two-hour intervals over eight hours indicated that enhanced Rac activity selectively increased branch additions and retractions, as did Cdc42 to a lesser extent. Activation of endogenous RhoA decreased branch extension without affecting branch additions and retractions, whereas dominant-negative RhoA increased branch extension. Finally, we provide data suggesting that RhoA mediates the promotion of normal dendritic arbor development by NMDA receptor activation.

Item Type: Paper
Uncontrolled Keywords: IN-VIVO CEREBRAL-CORTEX AXON GUIDANCE RAC KINASE FAMILY PHOSPHORYLATION SYNAPTOGENESIS FILOPODIA DYNAMICS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTPase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Rho
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
CSHL labs > Van Aelst lab
Depositing User: Matt Covey
Date Deposited: 18 Dec 2013 21:40
Last Modified: 18 Dec 2013 21:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29103

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