A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts

Minshull, J., Sun, H., Tonks, N. K., Murray, A. W. (November 1994) A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts. Cell, 79 (3). pp. 475-486. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/7954813
DOI: 10.1016/0092-8674(94)90256-9

Abstract

Like early Xenopus embryos, extracts made from Xenopus eggs lack the cell cycle checkpoint that keeps anaphase from occurring before spindle assembly is complete. At very high densities of sperm nuclei, however, microtubule depolymerization arrests the extracts in mitosis. The arrested extracts have high levels of maturation-promoting factor activity, fail to degrade cyclin B, and contain activated ERK2/mitogen-activated protein (MAP) kinase. The addition of the purified MAP kinase-specific phosphatase MKP-1 demonstrates that MAP kinase activity is required for both the establishment and maintenance of the mitotic arrest induced by spindle depolymerization. Increased calcium concentrations, which release unfertilized frog eggs from their natural arrest in metaphase of meiosis II, have no effect on the mitotic arrest.

Item Type: Paper
Uncontrolled Keywords: ACTIVATED PROTEIN-KINASE CELL-CYCLE ARREST WILD-TYPE P53 MATURATION-PROMOTING FACTOR IMMEDIATE-EARLY GENE M-PHASE SACCHAROMYCES-CEREVISIAE TYROSINE PHOSPHORYLATION MICROTUBULE DYNAMICS CHROMOSOME MOVEMENT
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > mitotic spindle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Tonks lab
Depositing User: Matt Covey
Date: November 1994
Date Deposited: 18 Dec 2013 15:30
Last Modified: 18 Dec 2013 15:30
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29067

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving