Cloning and expression of the chicken protein tyrosine phosphatase SH-PTP2

Park, C. Y., La Montagne, K. R., Tonks, N. K., Hayman, M. J. (October 1996) Cloning and expression of the chicken protein tyrosine phosphatase SH-PTP2. Gene, 177 (1-2). pp. 93-97. ISSN 0378-1119

URL: http://www.ncbi.nlm.nih.gov/pubmed/8921851
DOI: 10.1016/0378-1119(96)00278-8

Abstract

SH-PTP2 is a protein tyrosine phosphatase which contains two src homology 2 (SH2) domains. A partial cDNA clone encoding chicken SH-PTP2 was generated by RT-PCR and used as a probe to screen several chicken cDNA libraries. Two overlapping cDNA clones were identified and the nucleotide sequence of chicken SH-PTP2 containing the entire protein-coding region was determined. The deduced amino acid sequence shares 98% and 94% identity, respectively with the corresponding human and Xenopus proteins. Northern and Western blot analyses show that chicken SH-PTP2 is expressed ubiquitously like those of mammals and Xenopus. This suggests that chicken SH-PTP2 may have analogous biological roles to those of mammals.

Item Type: Paper
Uncontrolled Keywords: protein tyrosine phosphatase cDNA avian erythrocyte homology to mammalian SH-PTP2 SH2-CONTAINING PHOSPHOTYROSINE PHOSPHATASE SYP PHOSPHATASE KINASE CELLS CORKSCREW DOMAINS SIGNAL KIT
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein tyrosine phosphatase
CSHL Authors:
Communities: CSHL labs > Tonks lab
Depositing User: Matt Covey
Date: October 1996
Date Deposited: 18 Dec 2013 16:44
Last Modified: 18 Dec 2013 16:44
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29052

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