Association of PTP-PEST with the SH3 domain of p130(cas); a novel mechanism of protein tyrosine phosphatase substrate recognition

Garton, A. J., Burnham, M. R., Bouton, A. H., Tonks, N. K. (August 1997) Association of PTP-PEST with the SH3 domain of p130(cas); a novel mechanism of protein tyrosine phosphatase substrate recognition. Oncogene, 15 (8). pp. 877-885. ISSN 0950-9232

Abstract

The protein tyrosine phosphatase PTP-PEST displays remarkable substrate specificity, in vitro and in vivo for p130(cas) a signalling intermediate implicated in mitogenic signalling, cell-adhesion induced signalling, and in transformation by a variety of oncogenes. We have identified a high affinity interaction between the SH3 domain of p130(cas) and a proline-rich sequence ((PPPKPPR)-P-335) within the C-terminal segment of PTP-PEST. Mutation of proline 337 within this sequence to alanine significantly impairs the ability of PTP-PEST to recognise tyrosine phosphorylated p130(cas) as a substrate, without qualitatively affecting the selectivity of the interaction. Thus the highly specific nature of the interaction between PTP-PEST and p130(cas) appears to result from a combination of two distinct substrate recognition mechanisms; the catalytic domain of PTP-PEST contributes specificity to the interaction with p130(cas), whereas the SH3 domain-mediated association of p130(cas) and PTP-PEST dramatically increases the efficiency of the interaction. Furthermore, our results indicate that one important function of the p130(cas) SH3 domain is to associate with PTP-PEST and thereby facilitate the dephosphorylation of p130(cas), resulting in the termination of tyrosine phosphorylation-dependent signalling events downstream of p130(cas).

Item Type:	Paper
Uncontrolled Keywords:	p130(cas) SH3 domain PTP-PEST tyrosine phosphorylation PTP FOCAL ADHESION KINASE SWISS 3T3 CELLS V-CRK ONCOGENE GROWTH-FACTOR STRUCTURAL BASIS RECEPTOR-BETA CROSS-TALK PHOSPHORYLATION PHOSPHOTYROSINE PAXILLIN
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > p130 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > SH3 domain bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein tyrosine phosphatase
CSHL Authors:	Garton, Andrew Tonks, Nicholas K.
Communities:	CSHL labs > Tonks lab
Depositing User:	Matt Covey
Date:	August 1997
Date Deposited:	18 Dec 2013 15:59
Last Modified:	18 Dec 2013 15:59
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/29044

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