Arabidopsis thaliana DNA methylation mutants

Vongs, A., Kakutani, T., Martienssen, R. A., Richards, E. J. (1993) Arabidopsis thaliana DNA methylation mutants. Science, 260 (5116). pp. 1926-1928. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/8316832
DOI: 10.1126/science.8316832

Abstract

Three DNA hypomethylation mutants of the flowering plant Arabidopsis thaliana were isolated by screening mutagenized populations for plants containing centromeric repetitive DNA arrays susceptible to digestion by a restriction endonuclease that was sensitive to methylated cytosines. The mutations are recessive, and at least two are alleles of a single locus, designated DDM1 (for decrease in DNA methylation). Amounts of 5-methylcytosine were reduced over 70 percent in ddm1 mutants. Despite this reduction in DNA methylation levels, ddm1 mutants developed normally and exhibited no striking morphological phenotypes. However, the ddm1 mutations are associated with a segregation distortion phenotype. The ddm1 mutations were used to demonstrate that de novo DNA methylation in vivo is slow.

Item Type: Paper
Uncontrolled Keywords: sequence specificity transposable elements chromatin structure gene-expression maize methyltransferases inactivation cells ac
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date Deposited: 12 Dec 2013 19:31
Last Modified: 12 Dec 2013 19:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29006

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