Analysis of splice donor and acceptor site function in a transposable gene trap derived from the maize element Activator

Nussaume, L., Harrison, K., Klimyuk, V., Martienssen, R., Sundaresan, V., Jones, J. D. G. (November 1995) Analysis of splice donor and acceptor site function in a transposable gene trap derived from the maize element Activator. Molecular & General Genetics, 249 (1). pp. 91-101. ISSN 0026-8925

URL: http://www.ncbi.nlm.nih.gov/pubmed/8552039
DOI: 10.1007/bf00290240

Abstract

Gene trap vectors have been used in insertional mutagenesis in animal systems to clone genes with interesting patterns of expression. These vectors are designed to allow the expression of a reporter gene when the vector inserts into a transcribed region. In this paper we examine alternative splicing events that result in the expression of a GUS reporter gene carried on a Ds element which has been designed as a gene trap vector for plants. We have developed a rapid and reliable method based on PCR to study such events. Many splice donor sites were observed in the 3' Ac border. The relative frequency of utilisation of certain splice donor and acceptor sites differed between tobacco and Arabidopsis. A higher stringency of splicing was observed in Arabidopsis.

Item Type: Paper
Uncontrolled Keywords: splicing transposon tagging intron ac/ds developmentally regulated genes camv 35s-enhancer subdomains tissue-specific expression pre-messenger rnas arabidopsis-thaliana beta-glucuronidase insitu detection dicot plants transposition ac
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > introns
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons > transposon tagging
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: November 1995
Date Deposited: 12 Dec 2013 20:21
Last Modified: 12 Dec 2013 20:21
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28997

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