Functional genomics: Probing plant gene function and expression with transposons

Martienssen, R. A. (March 1998) Functional genomics: Probing plant gene function and expression with transposons. Proceedings of the National Academy of Sciences of the United States of America, 95 (5). pp. 2021-2026. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/9482828
DOI: 10.1073/pnas.95.5.2021

Abstract

Transposable elements provide a convenient and flexible means to disrupt plant genes, so allowing their function to be assessed. By engineering transposons to carry reporter genes and regulatory signals, the expression of target genes can be monitored and to some extent manipulated. Two strategies for using transposons to assess gene function are outlined here: First, the PCR can be used to identify plants that carry insertions into specific genes from among pools of heavily mutagenized individuals (site-selected transposon mutagenesis). This method requires that high copy transposons be used and that a relatively large number of reactions be performed to identify insertions into genes of interest. Second, a large library of plants, each carrying a unique insertion, can be generated. Each insertion site then can be amplified and sequenced systematically. These two methods have been demonstrated in maize, Arabidopsis, and other plant species, and the relative merits of each are discussed in the context of plant genome research.

Item Type: Paper
Uncontrolled Keywords: arabidopsis-thaliana caenorhabditis-elegans zea-mays maize elements dna insertion drosophila mutagenesis identification
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: March 1998
Date Deposited: 12 Dec 2013 19:56
Last Modified: 12 Dec 2013 19:56
PMCID: PMC33836
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28993

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