Independent deposition of heterogeneous nuclear ribonucleoproteins and small nuclear ribonucleoprotein particles at sites of transcription

Amero, S. A., Raychaudhuri, G., Cass, C. L., Vanvenrooij, W. J., Habets, W. J., Krainer, A. R., Beyer, A. L. (September 1992) Independent deposition of heterogeneous nuclear ribonucleoproteins and small nuclear ribonucleoprotein particles at sites of transcription. Proceedings of the National Academy of Sciences of the United States of America, 89 (18). pp. 8409-8413. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/1388268
DOI: 10.1073/pnas.89.18.8409

Abstract

The major nuclear ribonucleoproteins (RNPs) involved in pre-mRNA processing are classified in broad terms either as small nuclear RNPs (snRNPs), which are major participants in the splicing reaction, or heterogeneous nuclear RNPs (hnRNPs), which traditionally have been thought to function in general pre-mRNA packaging. We obtained antibodies that recognize these two classes of RNP in Drosophila melanogaster. Using a sequential immunostaining technique to compare directly the distribution of these RNPs on Drosophila polytene chromosomes, we found that the two patterns were very similar qualitatively but not quantitatively, arguing for the independent deposition of the two RNP types and supporting a role for hnRNP proteins, but not snRNPs, in general transcript packaging.

Item Type: Paper
Uncontrolled Keywords: messenger rna splicing rna processing pre-messenger-rna drosophila-melanogaster secondary structure mammalian nuclei hnrnp proteins human u1 u2 gene autoantibodies spliceosomes
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: September 1992
Date Deposited: 10 Dec 2013 20:19
Last Modified: 10 Dec 2013 20:19
PMCID: PMC49929
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28958

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