Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing

Mayeda, A., Zahler, A. M., Krainer, A. R., Roth, M. B. (February 1992) Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing. Proceedings of the National Academy of Sciences of the United States of America, 89 (4). pp. 1301-1304. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/1741384
DOI: 10.1073/pnas.89.4.1301

Abstract

Monoclonal antibody 104 recognizes a subset of amphibian nuclear granules (B-snurposomes) and active sites of RNA polymerase II transcription in vertebrates and invertebrates. Monoclonal antibody 104 reacts with a set of nuclear serine- and arginine-rich phosphoproteins (SR family) with strikingly conserved apparent molecular masses. The most abundant family members in human (SRp33) and Drosophila (SRp55) cell lines can replace one another as essential splicing factors in a human cell-free system. Each of these polypeptides can functionally replace human SF2, an essential splicing factor that also regulates 5' splice site selection of alternatively spliced pre-mRNAs in vitro. Drosophila SRp55 also functions as an alternative splicing factor in the human cell-free system. Analysis of cloned cDNAs shows that SRp55 and SF2 are highly related and reveals regions of similarity to genetically defined regulators of alternative splicing in Drosophila. These results suggest that the conserved SR family of phosphoproteins, which includes SRp55 and SF2, is involved in constitutive pre-mRNA splicing and in the specificity of alternative splice site selection.

Item Type: Paper
Uncontrolled Keywords: binding proteins drosophila-melanogaster site selection snrnp protein gene expression invitro u1 factor-sf2 similarity
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: February 1992
Date Deposited: 10 Dec 2013 20:15
Last Modified: 10 Dec 2013 20:15
PMCID: PMC48437
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28956

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving