U1-mediated exon definition interactions between AT-AC and GT-AG introns

Wu, Q., Krainer, A. R. (November 1996) U1-mediated exon definition interactions between AT-AC and GT-AG introns. Science, 274 (5289). pp. 1005-1008. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/8875927
DOI: 10.1126/science.274.5289.1005

Abstract

A minor class of metazoan introns has well-conserved splice sites with 5'-AU-AC-3' boundaries, compared to the 5'-GU-AG-3' boundaries and degenerate splice sites of conventional introns. Splicing of the AT-AC intron 2 of a sodium channel (SCN4A) precursor messenger RNA in vitro did not require inhibition of conventional splicing and required adenosine triphosphate, magnesium, and U12 small nuclear RNA (snRNA). When exon 3 was followed by the 5' splice site from the downstream conventional intron, splicing of intron 2 was greatly stimulated. This effect was U1 snRNA-dependent, unlike the basal AT-AC splicing reaction. Therefore, U1-mediated exon definition interactions can coordinate the activities of major and minor spliceosomes.

Item Type: Paper
Uncontrolled Keywords: pre-messenger-rna small nuclear ribonucleoproteins splice-site selection cartilage matrix protein sodium-channel gene genomic structure last nucleotides u1 snrnp invitro binding
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > introns
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: November 1996
Date Deposited: 10 Dec 2013 21:47
Last Modified: 10 Dec 2013 21:47
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28943

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving