A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs

Horowitz, D. S., Kobayashi, R., Krainer, A. R. (December 1997) A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs. Rna-a Publication of the Rna Society, 3 (12). pp. 1374-1387. ISSN 1355-8382

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URL: http://www.ncbi.nlm.nih.gov/pubmed/9404889

Abstract

We have purified three new human U4/U6-snRNP proteins from HeLa cells. The three proteins formed a tightly bound complex and behaved as a single species throughout the purification. All three proteins have been identified by peptide sequencing, and full-length cDNA sequences have been obtained for all of them. Two of the proteins are homologues of the Saccharomyces cerevisiae splicing factors Prp3 and Prp4, and the third protein is a cyclophilin. Both the human and S. cerevisiae Prp4 proteins have seven repeats of the WD motif and likely fold into structures very similar to those of the beta subunits of G proteins. The human Prp3 protein is highly basic and is closely related to S. cerevisiae Prp3 only in its carboxyl-terminal half. The human homologues of Prp3 and Prp4 are part of a stable complex in the absence of RNA. The third protein in the complex is a new cyclophilin. Cyclophilins have been proposed to act as chaperones in a variety of cellular processes, and we discuss some possible roles of this U4/U6 snRNP-associated cyclophilin.

Item Type: Paper
Uncontrolled Keywords: pre-mRNA splicing USA-CyP WD motif small nuclear ribonucleoprotein rna-rna interactions cis-trans isomerase saccharomyces-cerevisiae protein family splicing reaction crystal-structure binding-protein polymerase-ii cyclosporin-a
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Kobayashi lab
CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: December 1997
Date Deposited: 11 Dec 2013 16:30
Last Modified: 11 Dec 2013 16:30
PMCID: PMC1369579
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28941

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