Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing

Burns, C. G., Ohi, R., Krainer, A. R., Gould, K. L. (November 1999) Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Proceedings of the National Academy of Sciences of the United States of America, 96 (24). pp. 13789-13794. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/10570151
DOI: 10.1073/pnas.96.24.13789

Abstract

The conserved CDC5 family of Myb-related proteins performs an essential function in cell cycle control at G(2)/M. Although c-Myb and many Myb-related proteins act as transcription factors, herein, we implicate CDC5 proteins in pre-mRNA splicing. Mammalian CDC5 colocalizes with pre-mRNA splicing factors in the nuclei of mammalian cells, associates with core components of the splicing machinery in nuclear extracts, and interacts with the spliceosome throughout the splicing reaction in vitro. Furthermore, genetic depletion of the homolog of CDC5 in Saccharomyces cerevisiae, CEF1. blocks the first step of pre-mRNA processing in vivo. These data provide evidence that eukaryotic cells require CDC5 proteins for pre-mRNA splicing.

Item Type: Paper
Uncontrolled Keywords: yeast saccharomyces-cerevisiae cell-division cycle schizosaccharomyces-pombe fission yeast gene encodes mutants nucleus dna organization
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: November 1999
Date Deposited: 11 Dec 2013 15:03
Last Modified: 11 Dec 2013 15:03
PMCID: PMC24143
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28931

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