hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing

Caputi, M., Mayeda, A., Krainer, A. R., Zahler, A. M. (July 1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO Journal, 18 (14). pp. 4060-4067. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/10406810
DOI: 10.1093/emboj/18.14.4060

Abstract

Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are necessary for HIV-1 splicing inhibition. We demonstrate that heterogeneous nuclear ribonucleoproteins (hnRNPs) of the A and B group are trans-acting factors required for the function of the tnt exon 2 ESS, Depletion of hnRNP A/B proteins from HeLa cell nuclear extract activates splicing of mt exon 2 pre-mRNA substrate. Splicing inhibition is restored by addition of recombinant hnRNP A/B proteins to the depleted extract. A high-affinity hnRNP A1-binding sequence can substitute functionally for the ESS in tnt exon 2, These results demonstrate that hnRNP A/B proteins are required for repression of HIV-1 splicing.

Item Type: Paper
Uncontrolled Keywords: HIV-1 hnRNP proteins pre-mRNA splicing immunodeficiency-virus type-1 messenger-rna binding-proteins sr proteins tat exon-2 a1 expression silencer selection elements
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: July 1999
Date Deposited: 11 Dec 2013 19:31
Last Modified: 11 Dec 2013 19:31
PMCID: PMC1171481
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28930

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