Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins

Ismaili, N., Perez-Morga, D., Walsh, P., Mayeda, A., Pays, A., Tebabi, P., Krainer, A. R., Pays, E. (1999) Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins. Molecular and Biochemical Parasitology, 102 (1). pp. 103-115. ISSN 0166-6851

URL: http://www.ncbi.nlm.nih.gov/pubmed/10477180
DOI: 10.1016/s0166-6851(99)00091-2

Abstract

The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery from higher eukaryotes. Moreover, in the yeast two-hybrid system, TSR1 is able to interact with the human splicing factors involved in the recognition of the 3' splicing site (U2AF35/U2AF65). In both procyclic and bloodstream forms of T. brucei, TSR1 was found to localize in the nucleus. In the bloodstream stage TSR1 showed the speckles pattern characteristic of SR proteins involved in cis-splicing. Moreover, TSR1 was able to specifically bind the spliced leader (SL) RNA involved in trans-splicing in trypanosomes by the yeast three-hybrid system. These and other observations suggest that TSR1 may be involved in traits-splicing in T. brucei. (C) 1999 Elsevier Science B.V. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: Trypanosoma brucei trans-splicing SR protein RRM motif spliced leader RNA pre-messenger-rna small nuclear ribonucleoproteins intracellular-localization site selection trans leader recognition expression domain identification
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNA binding protein
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date Deposited: 11 Dec 2013 19:23
Last Modified: 11 Dec 2013 19:23
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28927

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