Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing

Mayeda, A., Badolato, J., Kobayashi, R., Zhang, M. Q., Gardiner, E. M., Krainer, A. R. (August 1999) Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing. EMBO Journal, 18 (16). pp. 4560-4570. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/10449421
DOI: 10.1093/emboj/18.16.4560

Abstract

Biochemical purification of a pre-mRNA splicing activity from HeLa cells that stimulates distal alternative 3' splice sites in a concentration-dependent manner resulted in the identification of RNPS1, a novel general activator of pre-mRNA splicing. RNPS1 cDNAs, encoding a putative nucleic-acid-binding protein of unknown function, were previously identified in mouse and human. RNPS1 is conserved in metazoans and has an RNA-recognition moth preceded by an extensive serine-rich domain. Recombinant human RNPS1 expressed in baculovirus functionally synergizes with SR proteins and strongly activates splicing of both constitutively and alternatively spliced pre-mRNAs, We conclude that RNPS1 is not only a potential regulator of alternative splicing but may also play a more fundamental role as a general activator of pre-mRNA splicing.

Item Type: Paper
Uncontrolled Keywords: alternative splicing RNA-recognition motif RNPS1 splicing activator splicing factor site selection in-vivo rna protein expression binding factor-sf2 sequence domains transcript
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:
Communities: CSHL labs > Kobayashi lab
CSHL labs > Krainer lab
CSHL labs > Zhang lab
Depositing User: Matt Covey
Date: August 1999
Date Deposited: 11 Dec 2013 17:37
Last Modified: 11 Dec 2013 17:37
PMCID: PMC1171530
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28924

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