The type 2C Ser/Thr phosphatase PP2C gamma is a pre-mRNA splicing factor

Murray, M. V., Kobayashi, R., Krainer, A. R. (January 1999) The type 2C Ser/Thr phosphatase PP2C gamma is a pre-mRNA splicing factor. Genes & Development, 13 (1). pp. 87-97. ISSN 0890-9369

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URL: http://www.ncbi.nlm.nih.gov/pubmed/9887102
DOI: 10.1101/gad.13.1.87

Abstract

To identify activities involved in human pre-mRNA splicing, we have developed a procedure to separate HeLa cell nuclear extract into five complementing fractions. An activity called SCF1 was purified from one of these fractions by assaying for reconstitution of splicing in the presence of the remaining four fractions. A component of SCF1 is shown to be PP2C gamma, a type 2C Ser/Thr phosphatase of previously unknown function. Previous work suggested that dephosphorylation of splicing factors may be important for catalysis after spliceosome assembly, although the identities of the specific phosphatases involved remain unclear. Here we show that human PP2C gamma is physically associated with the spliceosome in vitro throughout the splicing reaction, but is first required during the early stages of spliceosome assembly for efficient formation of the A complex. The phosphatase activity is required for the splicing function of PP2C gamma, as an active site mutant does not support spliceosome assembly. The requirement for PP2C gamma is highly specific, as the closely related phosphatase PP2C alpha cannot substitute for PP2C gamma. Consistent with a role in splicing, PP2C gamma localizes to the nucleus in vivo. We conclude that at least one specific dephosphorylation event catalyzed by PP2C gamma is required for formation of the spliceosome.

Item Type: Paper
Uncontrolled Keywords: PP2C gamma phosphatase splicing pre-mRNA processing spliceosome serine-threonine phosphatase human protein phosphatase messenger-rna sr proteins molecular-cloning cell-cycle phosphorylation kinase spliceosome purification
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein phosphatase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Krainer lab
CSHL labs > Kobayashi lab
Depositing User: Matt Covey
Date: January 1999
Date Deposited: 11 Dec 2013 16:56
Last Modified: 11 Dec 2013 16:56
PMCID: PMC316367
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28922

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