Human p32: A coactivator for Epstein-Barr virus nuclear antigen-1-mediated transcriptional activation and possible role in viral latent cycle DNA replication

Van Scoy, S., Watakabe, I., Krainer, A. R., Hearing, J. (September 2000) Human p32: A coactivator for Epstein-Barr virus nuclear antigen-1-mediated transcriptional activation and possible role in viral latent cycle DNA replication. Virology, 275 (1). pp. 145-157. ISSN 0042-6822

URL: http://www.ncbi.nlm.nih.gov/pubmed/11017796
DOI: 10.1006/viro.2000.0508

Abstract

The Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) is required for the maintenance of the viral chromosome in latently infected, proliferating cells and plays a role in latent cycle DNA replication. EBNA-1 also functions as a positive and negative regulator of EBV gene expression. We have investigated the interaction of EBNA-1 with p32, a host mitochondrial protein that associates with EBNA-1 in EBV-positive Burkitt's lymphoma cells. Using a chromatin immunoprecipitation assay, we found that a fraction of p32 localizes to the viral latent cycle origin of DNA replication oriP in vivo, p32 binds EBNA-1 independently of other proteins or DNA. EBNA-1 variants lacking one of two p32 binding elements did not interact stably with p32 in cultured cells and were defective for both transcriptional activation of a reporter gene linked to oriP FR and replication and/or maintenance of a plasmid bearing oriP These results support a role for p32 in transcriptional activation by EBNA-1 and suggest that p32 plays a role in EBV latent cycle DNA replication. (C) 2000 Academic Press.

Item Type: Paper
Uncontrolled Keywords: origin-binding-protein type-1 tat transactivator in-vitro interaction cellular protein mitochondrial matrix rna-binding saccharomyces-cerevisiae metaphase chromosomes stable replication ebna1
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > virus
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: September 2000
Date Deposited: 11 Dec 2013 19:53
Last Modified: 11 Dec 2013 19:53
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28914

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