MHF1-2/CENP-S-X performs distinct roles in centromere metabolism and genetic recombination

Bhattacharjee, S., Osman, F., Feeney, L., Lorenz, A., Bryer, C., Whitby, M. C. (September 2013) MHF1-2/CENP-S-X performs distinct roles in centromere metabolism and genetic recombination. Open Biology, 3 (SEP). ISSN 20462441 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/24026537
DOI: 10.1098/rsob.130102

Abstract

The histone-fold proteins Mhf1/CENP-S and Mhf2/CENP-X perform two important functions in vertebrate cells. First, they are components of the constitutive centromere-associated network, aiding kinetochore assembly and function. Second, they work with the FANCM DNA translocase to promote DNA repair. However, it has been unclear whether there is crosstalk between these roles. We showthat Mhf1 and Mhf2 in fission yeast, as in vertebrates, serve a dual function, aiding DNA repair/recombination and localizing to centromeres to promote chromosome segregation. Importantly, these functions are distinct, with the former being dependent on their interaction with the FANCM orthologue Fml1 and the latter not. Together with Fml1, they play a second role in aiding chromosome segregation by processing sister chromatid junctions. However, a failure of this activity does not manifest dramatically increased levels of chromosome missegregation due to the Mus81-Eme1 endonuclease, which acts as a failsafe to resolve DNA junctions before the end of mitosis. © 2013 The Authors.

Item Type: Paper
Uncontrolled Keywords: Anaphase bridge Centromere DNA helicase DNA replication Homologous recombination
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > recombination
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: 11 September 2013
Date Deposited: 11 Nov 2013 21:36
Last Modified: 11 Nov 2013 21:36
PMCID: PMC3787749
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28850

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