The tumor suppressor Chd5 is induced during neuronal differentiation, in the developing mouse brain

Vestin, A., Mills, A. A. (October 2013) The tumor suppressor Chd5 is induced during neuronal differentiation, in the developing mouse brain. Gene Expression Patterns, 13 (8). pp. 482-489. ISSN 1872-7298 (Electronic)1567-133X (Linking)

Abstract

Epigenetic regulation of gene expression orchestrates dynamic cellular processes that become perturbed in human disease. An understanding of how subversion of chromatin-mediated events leads to pathologies such as cancer and neurodevelopmental syndromes may offer better treatment options for these pathological conditions. Chromodomain Helicase DNA-binding protein 5 (CHD5) is a dosage-sensitive tumor suppressor that is inactivated in human cancers, including neural-associated malignancies such as neuroblastoma and glioma. Here we report a detailed analysis of the temporal and cell type-specific expression pattern of Chd5 in the mammalian brain. By analyzing endogenous Chd5 protein expression during mouse embryogenesis, in the neonate, and in the adult, we found that Chd5 is expressed broadly in multiple brain regions, that Chd5 sub-cellular localization undergoes a switch from the cytoplasm to the nucleus during mid-gestation, and that Chd5 expression is retained at high levels in differentiated neurons of the adult. These findings may have important implications for defining the role of CHD5-mediated chromatin dynamics in the brain and for elucidating how perturbation of these epigenetic processes leads to neuronal malignancies, neurodegenerative diseases, and neurodevelopmental syndromes.

Item Type:	Paper
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Chd5 organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > tumor suppressor
CSHL Authors:	Mills, Alea A. Vestin, Assaf
Communities:	CSHL Cancer Center Program > Cancer Genetics CSHL Cancer Center Shared Resources > Animal Services CSHL Cancer Center Shared Resources > Gene Targeting Service CSHL Cancer Center Shared Resources > Microscopy Service CSHL labs > Mills lab CSHL Cancer Center Shared Resources > Animal Tissue and Imaging Service
Depositing User:	Matt Covey
Date:	8 October 2013
Date Deposited:	05 Nov 2013 15:47
Last Modified:	29 Oct 2015 20:43
PMCID:	PMC3855250
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28843

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