Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi

Volpe, T. A., Kidner, C., Hall, I. M., Teng, G., Grewal, S. I., Martienssen, R. A. (2002) Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi. Science, 297 (5588). pp. 1833-7. ISSN 1095-9203 (Electronic)0036-8075 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/12193640
DOI: 10.1126/science.1074973

Abstract

Eukaryotic heterochromatin is characterized by a high density of repeats and transposons, as well as by modified histones, and influences both gene expression and chromosome segregation. In the fission yeast Schizosaccharomyces pombe, we deleted the argonaute, dicer, and RNA-dependent RNA polymerase gene homologs, which encode part of the machinery responsible for RNA interference (RNAi). Deletion results in the aberrant accumulation of complementary transcripts from centromeric heterochromatic repeats. This is accompanied by transcriptional de-repression of transgenes integrated at the centromere, loss of histone H3 lysine-9 methylation, and impairment of centromere function. We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi.

Item Type: Paper
Uncontrolled Keywords: Centromere/physiology Chromosomal Proteins, Non-Histone/metabolism DNA Methylation DNA Transposable Elements Endoribonucleases/genetics/metabolism Gene Deletion *Gene Silencing Genes, Fungal Heterochromatin/genetics/*metabolism Histones/*metabolism Lysine/*metabolism Methylation Models, Genetic RNA Replicase/genetics/metabolism RNA, Double-Stranded/metabolism RNA, Fungal/metabolism RNA, Small Interfering RNA, Untranslated/genetics/*metabolism Repetitive Sequences, Nucleic Acid Ribonuclease III Schizosaccharomyces/*genetics/metabolism Schizosaccharomyces pombe Proteins/genetics/metabolism Transcription, Genetic Transgenes
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
CSHL Authors:
Communities: CSHL labs > Grewal lab
CSHL labs > Martienssen lab
Watson School > Publications
Depositing User: Matt Covey
Date Deposited: 28 Oct 2013 15:38
Last Modified: 19 Sep 2014 14:17
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28806

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