Stable and unstable pools of Myc protein exist in human cells

Tworkowski, K. A., Salghetti, S. E., Tansey, W. P. (December 2002) Stable and unstable pools of Myc protein exist in human cells. Oncogene, 21 (55). pp. 8515-8520. ISSN 0950-9232

URL: http://www.ncbi.nlm.nih.gov/pubmed/12466972
DOI: 10.1038/sj.onc.1205976

Abstract

The oncoprotein transcription factor Myc plays a crucial role in the control of cell growth and proliferation. Consistent with its potent growth-promoting properties, cells have evolved a number of mechanisms to limit the activity and accumulation of the Myc protein. One of the most striking of these mechanisms is ubiquitin (Ub)-mediated proteolysis, which typically destroys Myc within minutes of its synthesis. Here we show that, despite the extreme instability of the Myc protein, cells contain a pool of Myc that is metabolically stable. Entry of Myc into the stable pool is signaled by an element within the carboxy-terminus of the protein, and is a cell-specific process that is regulated during mitosis and by interaction with Max. These data demonstrate that - even for a rapidly turned-over protein such as Myc - metabolically stable and unstable forms of a protein can co-exist in cells, and suggest that the rate of destruction of Myc molecules is linked to their specific functions.

Item Type: Paper
Uncontrolled Keywords: Myc Max ubiquitin-mediated proteolysis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > Myc
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Tansey lab
Depositing User: Matt Covey
Date: December 2002
Date Deposited: 31 Oct 2013 14:09
Last Modified: 31 Oct 2013 14:09
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28800

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving