Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast

Nonaka, N., Kitajima, T., Yokobayashi, S., Xiao, G. P., Yamamoto, M., Grewal, S. I. S., Watanabe, Y. (2002) Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast. Nature Cell Biology, 4 (1). pp. 89-93. ISSN 1465-7392

URL: http://www.ncbi.nlm.nih.gov/pubmed/11780129
DOI: 10.1038/ncb739

Abstract

Fission yeast centromeres, like those of higher eukaryotes, are composed of repeated DNA structures and associated heterochromatin protein complexes, that have a critical function in the faithful segregation of chromosomes during cell division1, 2, 3. Cohesin protein complexes, which are essential for sister-chromatid cohesion and proper chromosome segregation, are enriched at centromeric repeats4, 5. We have identified a functional and physical link between heterochromatin and cohesin. We find that the preferential localization of cohesins at the centromeric repeats is dependent on Swi6, a conserved heterochromatin protein that is required for proper kinetochore function. Cohesin is also enriched at the mating-type heterochromatic region in a manner that depends on Swi6 and is required to preserve the genomic integrity of this locus. We provide evidence that a cohesin subunit Psc3 interacts with Swi6 and its mouse homologue HP1. These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting the proper segregation of chromosomes.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Grewal lab
Depositing User: Matt Covey
Date Deposited: 08 Jan 2014 14:55
Last Modified: 08 Jan 2014 14:55
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28758

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