Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast

Nonaka, N., Kitajima, T., Yokobayashi, S., Xiao, G. P., Yamamoto, M., Grewal, S. I. S., Watanabe, Y. (January 2002) Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast. Nature Cell Biology, 4 (1). pp. 89-93. ISSN 1465-7392

URL: http://www.ncbi.nlm.nih.gov/pubmed/11780129
DOI: 10.1038/ncb739

Abstract

Fission yeast centromeres, like those of higher eukaryotes, are composed of repeated DNA structures and associated heterochromatin protein complexes, that have a critical function in the faithful segregation of chromosomes during cell division1, 2, 3. Cohesin protein complexes, which are essential for sister-chromatid cohesion and proper chromosome segregation, are enriched at centromeric repeats4, 5. We have identified a functional and physical link between heterochromatin and cohesin. We find that the preferential localization of cohesins at the centromeric repeats is dependent on Swi6, a conserved heterochromatin protein that is required for proper kinetochore function. Cohesin is also enriched at the mating-type heterochromatic region in a manner that depends on Swi6 and is required to preserve the genomic integrity of this locus. We provide evidence that a cohesin subunit Psc3 interacts with Swi6 and its mouse homologue HP1. These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting the proper segregation of chromosomes.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Grewal lab
Depositing User: Matt Covey
Date: January 2002
Date Deposited: 08 Jan 2014 14:55
Last Modified: 08 Jan 2014 14:55
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28758

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