Targeted electroporation in Xenopus tadpoles in vivo - from single cells to the entire brain

Haas, K., Jensen, K., Sin, W. C., Foa, L., Cline, H. T. (June 2002) Targeted electroporation in Xenopus tadpoles in vivo - from single cells to the entire brain. Differentiation, 70 (4-5). pp. 148-154. ISSN 0301-4681

URL: http://www.ncbi.nlm.nih.gov/pubmed/12147134
DOI: 10.1046/j.1432-0436.2002.700404.x

Abstract

Electroporation is becoming more popular as a technique for transfecting neurons within intact tissues. One of the advantages of electroporation over other transfection techniques is the ability to precisely target an area for transfection. Here we highlight this advantage by describing methods to restrict transfection to either a single cell, clusters of cells, or to include large portions of the brain of the intact Xenopus tadpole. Electroporation is also an effective means of gene delivery in the retina. We have developed these techniques to examine the effects of regulated gene expression on various neuronal properties, including structural plasticity and synaptic transmission. Restriction of transfection to individual cells aids in imaging of neuronal morphology, while bulk cell transfection allows examination of the affects of gene expression on populations of cells by biochemical assays, imaging, and electrophysiological recording.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > brain
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
Investigative techniques and equipment > transfection > single cell electroporation
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Matt Covey
Date: June 2002
Date Deposited: 08 Jan 2014 17:50
Last Modified: 08 Jan 2014 17:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28710

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