Memory enhancement and formation by atypical PKM activity in Drosophila melanogaster

Drier, E. A., Tello, M. K., Cowan, M., Wu, P., Blace, N., Sacktor, T. C., Yin, J. C. P. (April 2002) Memory enhancement and formation by atypical PKM activity in Drosophila melanogaster. Nature Neuroscience, 5 (4). pp. 316-324. ISSN 1097-6256

URL: http://www.ncbi.nlm.nih.gov/pubmed/11914720
DOI: 10.1038/nn820

Abstract

Synaptic stimulation activates signal transduction pathways, producing persistently active protein kinases. PKMzeta is a truncated, persistently active isoform of atypical protein kinase C-zeta (aPKCzeta), which lacks the N-terminal pseudosubstrate regulatory domain. Using a Pavlovian olfactory learning task in Drosophila, we found that induction of the mouse aPKMzeta (MaPKMzeta) transgene enhanced memory. The enhancement required persistent kinase activity and was temporally specific, with optimal induction at 30 minutes after training. Induction also enhanced memory after massed training and corrected the memory defect of radish mutants, but did not improve memory produced by spaced training. The 'M' isoform of the Drosophila homolog of MaPKCzeta (DaPKM) was present and active in fly heads. Chelerythrine, an inhibitor of PKMzeta, and the induction of a dominant-negative MaPKMzeta transgene inhibited memory without affecting learning. Finally, induction of DaPKM after training also enhanced memory. These results show that atypical PKM is sufficient to enhance memory in Drosophila and suggest that it is necessary for normal memory maintenance.

Item Type: Paper
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organism description > animal behavior > memory
CSHL Authors:
Communities: CSHL labs > Yin lab
Dolan DNA Learning Center
Depositing User: Matt Covey
Date: April 2002
Date Deposited: 08 Jan 2014 20:38
Last Modified: 08 Jan 2014 20:38
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28698

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