Metal chelating properties of pyridine-2,6-bis(thiocarboxylic acid) produced by Pseudomonas spp. and the biological activities of the formed complexes

Cortese, M. S., Paszczynski, A., Lewis, T. A., Sebat, J. L., Borek, V., Crawford, R. L. (June 2002) Metal chelating properties of pyridine-2,6-bis(thiocarboxylic acid) produced by Pseudomonas spp. and the biological activities of the formed complexes. Biometals, 15 (2). pp. 103-20. ISSN 0966-0844

URL: http://www.ncbi.nlm.nih.gov/pubmed/12046919
DOI: 10.1023/A:1015241925322

Abstract

We evaluated the ability of pyridine-2,6-bis(thiocarboxylic acid) (pdtc) to form complexes with 19 metals and 3 metalloids. Pdtc formed complexes with 14 of the metals. Two of these metal:pdtc complexes, Co:(pdtc)2 and Cu:pdtc, showed the ability to cycle between redox states, bringing to 4 the number of known redox-active pdtc complexes. A precipitant formed when pdtc was added to solutions of As, Cd, Hg, Mn, Pb, and Se. Additionally, 14 of 16 microbial strains tested were protected from Hg toxicity when pdtc was present. Pdtc also mediated protection from the toxic effects of Cd and Te, but for fewer strains. Pdtc by itself does not facilitate iron uptake, but increases the overall level of iron uptake of Pseudomonas stutzeri strain KC and P. putida DSM301. Both these pseudomonads could reduce amorphous Fe(III) oxyhydroxide in culture. In vitro reactions showed that copper and pdtc were required for this activity. This reaction may derive its reducing power from the hydrolysis of the thiocarboxyl groups of pdtc.

Item Type: Paper
Uncontrolled Keywords: Bacteria/drug effects/growth & development Candida albicans/drug effects/growth & development Chelating Agents/*metabolism/*pharmacology Diagnostic Imaging Iron/metabolism Mass Spectrometry Metals, Heavy/*metabolism Microbial Sensitivity Tests Molecular Structure Oxidation-Reduction Pseudomonas/*chemistry Pyridines/*metabolism/*pharmacology
Subjects: organism description > bacteria
CSHL Authors:
Communities: CSHL labs > Sebat lab
Depositing User: Matt Covey
Date: June 2002
Date Deposited: 08 Jan 2014 21:40
Last Modified: 08 Jan 2014 21:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28690

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