Visually driven modulation of glutamatergic synaptic transmission is mediated by the regulation of intracellular polyamines

Aizenman, C. D., Munoz-Elias, G., Cline, H. T. (May 2002) Visually driven modulation of glutamatergic synaptic transmission is mediated by the regulation of intracellular polyamines. Neuron, 34 (4). pp. 623-634. ISSN 0896-6273

URL: http://www.ncbi.nlm.nih.gov/pubmed/12062045
DOI: 10.1016/S0896-6273(02)00674-8

Abstract

Ca2+-permeable AMPARs are inwardly rectifying due to block by intracellular polyamines. Neuronal activity regulates polyamine synthesis, yet whether this affects Ca2+-AMPAR-mediated synaptic transmission is unknown. We test whether 4 hr of increased visual stimulation regulates glutamatergic retino-tectal synapses in Xenopus tadpoles. Tectal neurons containing Ca2+-AMPARs form a gradient along the rostro-caudal developmental axis. These neurons had inwardly rectifying AMPAR-mediated EPSCs. Four hours of visual stimulation or addition of intracellular spermine increased rectification in immature neurons. Polyamine synthesis inhibitors blocked the effect of visual stimulation, suggesting that visual activity regulates AMPARs via the polyamine synthesis pathway. This modulation resulted in changes in the integrative properties of tectal neurons. Regulation of polyamine synthesis by physiological stimuli is a novel form of modulation of synaptic transmission important for understanding the short-term effects of enhanced sensory experience during development.

Item Type: Paper
Uncontrolled Keywords: Animals Biogenic Polyamines/*metabolism/pharmacology Calcium Signaling/drug effects/physiology Cell Differentiation/drug effects/*physiology Cell Membrane Permeability/drug effects/physiology Excitatory Amino Acid Antagonists/pharmacology Excitatory Postsynaptic Potentials/drug effects/physiology Glutamic Acid/*metabolism Ion Channels/drug effects/metabolism Larva Photic Stimulation Presynaptic Terminals/*metabolism/ultrastructure Receptors, AMPA/drug effects/metabolism Receptors, N-Methyl-D-Aspartate/drug effects/metabolism Retina/cytology/*growth & development/metabolism Spermine/metabolism/pharmacology Superior Colliculus/cytology/*growth & development/metabolism Synaptic Transmission/drug effects/*physiology Visual Pathways/cytology/*growth & development/metabolism Xenopus laevis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > small molecules > Glutamate
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Matt Covey
Date: May 2002
Date Deposited: 09 Jan 2014 15:48
Last Modified: 09 Jan 2014 15:48
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28657

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