Plant MicroRNAs Display Differential 3 ' Truncation and Tailing Modifications That Are ARGONAUTE1 Dependent and Conserved Across Species

Zhai, J. X., Zhao, Y. Y., Simon, S. A., Huang, S., Petsch, K., Arikit, S., Pillay, M., Ji, L. J., Xie, M., Cao, X. F., Yu, B., Timmermans, M., Yang, B., Chen, X. M., Meyers, B. C. (July 2013) Plant MicroRNAs Display Differential 3 ' Truncation and Tailing Modifications That Are ARGONAUTE1 Dependent and Conserved Across Species. Plant Cell, 25 (7). pp. 2417-2428. ISSN 1040-4651

URL: http://www.ncbi.nlm.nih.gov/pubmed/23839787
DOI: 10.1105/tpc.113.114603

Abstract

Plant small RNAs are 3' methylated by the methyltransferase HUA1 ENHANCER1 (HEN1). In plant hen1 mutants, 3' modifications of small RNAs, including oligo-uridylation (tailing), are associated with accelerated degradation of microRNAs (miRNAs). By sequencing small RNAs of the wild type and hen1 mutants from Arabidopsis thaliana, rice (Oryza sativa), and maize (Zea mays), we found 3' truncation prior to tailing is widespread in these mutants. Moreover, the patterns of miRNA truncation and tailing differ substantially among miRNA families but are conserved across species. The same patterns are also observable in wild-type libraries from a broad range of species, only at lower abundances. ARGONAUTE (AGO1), even with defective slicer activity, can bind these truncated and tailed variants of miRNAs. An ago1 mutation in hen1 suppressed such 3' modifications, indicating that they occur while miRNAs are in association with AGO1, either during or after RNA-induced silencing complex assembly. Our results showed AGO1-bound miRNAs are actively 3' truncated and tailed, possibly reflecting the activity of cofactors acting in conserved patterns in miRNA degradation.

Item Type: Paper
Uncontrolled Keywords: directed dna methylation small rnas arabidopsis-thaliana sirna biogenesis tal effectors target hen1 mirnas recognition degradation
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:
Communities: CSHL labs > Timmermans lab
Depositing User: Matt Covey
Date: July 2013
Date Deposited: 25 Sep 2013 15:53
Last Modified: 25 Sep 2013 15:53
PMCID: PMC3753374
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28630

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving