Monitoring synaptic plasticity by imaging AMPA receptor content and dynamics on dendritic spines

Makino, H., Li, B. (2013) Monitoring synaptic plasticity by imaging AMPA receptor content and dynamics on dendritic spines. Methods Mol Biol, 1018. pp. 269-275. ISSN 1940-6029 (Electronic)1064-3745 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/23681636
DOI: 10.1007/978-1-62703-444-9-25

Abstract

Time-lapse imaging techniques are widely used to monitor dendritic spine dynamics, a measurement of synaptic plasticity. However, it is challenging to follow the dynamics of spines over an extended period in vivo during development or in deep brain structures that are beyond the reach of traditional microscopes. Here, we describe an AMPA receptor-based optical approach to monitor recent history of synaptic plasticity. This method allows the identification of spines that have recently acquired synaptic AMPA receptors in a single imaging session, so that synaptic plasticity that occurs in vivo in a variety of conditions can be simply imaged in an ex vivo preparation. © 2013 Springer Science+Business Media, LLC.

Item Type: Paper
Uncontrolled Keywords: AMPA receptor trafficking Dendritic spine Fluorescence recovery after photobleaching Super Ecliptic pHluorin Synaptic plasticity Two-photon imaging
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
CSHL Authors:
Communities: CSHL labs > Li lab
Depositing User: Matt Covey
Date Deposited: 25 Sep 2013 15:45
Last Modified: 27 Jan 2017 20:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28627

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