Target specificity among canonical nuclear poly(A) polymerases in plants modulates organ growth and pathogen response

Vi, S. L., Trost, G., Lange, P., Czesnick, H., Rao, N., Lieber, D., Laux, T., Gray, W. M., Manley, J. L., Groth, D., Kappel, C., Lenhard, M. (August 2013) Target specificity among canonical nuclear poly(A) polymerases in plants modulates organ growth and pathogen response. Proceedings of the National Academy of Sciences of the United States of America, 110 (34). pp. 13994-13999. ISSN 00278424

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URL: http://www.ncbi.nlm.nih.gov/pubmed/23918356
DOI: 10.1073/pnas.1303967110

Abstract

Polyadenylation of pre-mRNAs is critical for efficient nuclear export, stability, and translation of the mature mRNAs, and thus for gene expression. The bulk of pre-mRNAs are processed by canonical nuclear poly(A) polymerase (PAPS). Both vertebrate and higher-plant genomes encode more than one isoform of this enzyme, and these are coexpressed in different tissues. However, in neither case is it known whether the isoforms fulfill different functions or polyadenylate distinct subsets of pre-mRNAs. Here we show that the three canonical nuclear PAPS isoforms in Arabidopsis are functionally specialized owing to their evolutionarily divergent C-terminal domains. A strong loss-of-function mutation in PAPS1 causes a male gametophytic defect, whereas a weak allele leads to reduced leaf growth that results in part from a constitutive pathogen response. By contrast, plants lacking both PAPS2 and PAPS4 function are viable with wild-type leaf growth. Polyadenylation of SMALL AUXIN UP RNA (SAUR) mRNAs depends specifically on PAPS1 function. The resulting reduction in SAUR activity in paps1 mutants contributes to their reduced leaf growth, providing a causal link between polyadenylation of specific pre-mRNAs by a particular PAPS isoform and plant growth. This suggests the existence of an additional layer of regulation in plant and possibly vertebrate gene expression, whereby the relative activities of canonical nuclear PAPS isoforms control de novo synthesized poly(A) tail length and hence expression of specific subsets of mRNAs.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > DNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organism description > plant
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: Matt Covey
Date: 20 August 2013
Date Deposited: 18 Sep 2013 15:54
Last Modified: 21 Dec 2017 15:45
PMCID: PMC3752211
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28581

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