The SIR1 gene of Saccharomyces cerevisiae and its role as an extragenic suppressor of several mating-defective mutants

Stone, E. M., Swanson, M. J., Romeo, A. M., Hicks, J. B., Sternglanz, R. (1991) The SIR1 gene of Saccharomyces cerevisiae and its role as an extragenic suppressor of several mating-defective mutants. Molecular and Cellular Biology, 11 (4). pp. 2253-2262. ISSN 02707306 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2005909

Abstract

The SIR1 gene product of Saccharomyces cerevisiae is one of several proteins involved in repressing transcription of the silent mating-type genes. Strains with mutations in the genes coding for these proteins are defective in mating due to derepression of the silent loci. We have found that overexpression of the SIR1 gene suppresses the mating defects of several of these mutants, including nat1 and ard1 mutants (the products of these two genes are responsible for N-terminal acetylation of a subset of yeast proteins), certain sir3 mutants, and a histone H4 mutant. The SIR1 gene has been sequenced and found to contain an open reading frame coding for a 678-amino-acid protein.

Item Type: Paper
Uncontrolled Keywords: article dna sequence fungus mutant gene structure mating nonhuman priority journal saccharomyces cerevisiae Amino Acid Sequence Base Sequence Gene Expression Regulation, Fungal Genes, Fungal Genes, Suppressor Genetic Complementation Test Histones Molecular Sequence Data Mutation Reproduction Restriction Mapping Support, U.S. Gov't, P.H.S. Transcription, Genetic Fungi
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1991
Date Deposited: 09 Aug 2013 20:46
Last Modified: 09 Aug 2013 20:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28524

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