Development of DNA probes for early diagnosis and epidemiological study of cryptococcosis in AIDS patients

Polacheck, I., Lebens, G., Hicks, J. B. (1992) Development of DNA probes for early diagnosis and epidemiological study of cryptococcosis in AIDS patients. Journal of Clinical Microbiology, 30 (4). pp. 925-930. ISSN 00951137 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1572979

Abstract

We report the isolation of middle-repetitive DNA sequences from Cryptococcus neoformans that are species and variety specific. These probes were used for assessing strain relatedness among cryptococcal isolates from patients with and without AIDS who were from Zaire and the United States. Five distinct hybridization patterns were observed for the 60 isolates examined, regardless of the restriction enzyme used for digestion. The most common pattern among the isolates from the patients without AIDS was also the most common among the isolates from the patients with AIDS who were from the United States and was the only pattern observed for all isolates tested from patients with AIDS who were from Zaire. On the basis of the high specificity and sensitivity of the signals observed by hybridization, we suggest that these sequences provide a means for both biotyping and early diagnosis of C. neoformans.

Item Type: Paper
Uncontrolled Keywords: restriction endonuclease acquired immune deficiency syndrome agar gel electrophoresis article cryptococcosis cryptococcus neoformans dna binding dna probe human hybridization isotope labeling plasmid priority journal southern blotting strain difference Acquired Immunodeficiency Syndrome Bacterial Typing Techniques Bacteriological Techniques Base Sequence Cryptococcus DNA Probes DNA, Bacterial Evaluation Studies Molecular Probe Techniques Molecular Sequence Data Opportunistic Infections Sensitivity and Specificity Species Specificity
Subjects: diseases & disorders
Investigative techniques and equipment > probes > DNA probes
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1992
Date Deposited: 09 Aug 2013 20:56
Last Modified: 12 Aug 2013 14:41
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28507

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