Genetic differences between type I and type II Candida stellatoidea

Kwon-Chung, K. J., Riggsby, W. S., Uphoff, R. A., Hicks, J. B., Whelan, W. L., Reiss, E., Magee, B. B., Wickes, B. L. (1989) Genetic differences between type I and type II Candida stellatoidea. Infection and Immunity, 57 (2). pp. 527-532. ISSN 00199567 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2643573

Abstract

Genetic similarities and differences between type I and type II Candida stellatoidea were studied. The electrophoretic karyotype, mitochondrial DNA (mtDNA) restriction patterns, and midrepeat sequence of nuclear DNA in type I C. stellatoidea were clearly distinguishable from those of a reference culture of Candida albicans. The karyotype and the major bands of the midrepeat sequence of type II C. stellatoidea were indistinguishable from those of the reference C. albicans. The mtDNA restriction patterns of four type I isolates were homogeneous regardless of the endonucleases and probes used. The mtDNA restriction patterns of type II C. stellatoidea varied from strain to strain. Some of them were identical to that of C. albicans, while others were the same as that of type I C. stellatoidea. Immunofluorescence with C. albicans serotype A-specific monoclonal antibody indicated that the four isolates of type I C. stellatoidea were serotype B (non-A), whereas all three type II isolates studied were serotype A. Taken together, these results support the hypothesis that the isolates of C. stellatoidea type II studied are sucrose-negative mutants of serotype A C. albicans. Since C. stellatoidea type I differs from C. albicans in several major genetic characteristics, it cannot be viewed as a simple mutant derived from C. albicans. Hybrids produced by protoplast fusion of type I and type II cells were capable of assimilating sucrose, indicating that the sucrose-negative phenotypes of the parents are due to different mutations.

Item Type: Paper
Uncontrolled Keywords: candida stellatoidea fungus gene structure nonhuman priority journal Candida Candida albicans Comparative Study Electrophoresis, Agar Gel Genetic Complementation Test Karyotyping Repetitive Sequences, Nucleic Acid Serotyping
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1989
Date Deposited: 09 Aug 2013 20:55
Last Modified: 12 Aug 2013 14:56
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28499

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