Precise mapping of the homothallism genes HML and HMR in Saccharomyces cerevisiae

Klar, A. J. S., McIndoo, J., Hicks, J. B., Strathern, J. N. (1980) Precise mapping of the homothallism genes HML and HMR in Saccharomyces cerevisiae. Genetics, 96 (2). pp. 315-320. ISSN 00166731 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/6790330

Abstract

The HML and HMR loci carry unexpressed copies of MATa and MAT alpha information, and a replica of that information is transposed to MAT during mating-type interchange in Saccharomyces yeasts. A negative control mechanism keeps silent the information located at the HML and HMR loci. We mapped these loci by constructing strains in which these loci are expressed. In these strains, the mating type of the segregants is dependent upon the allele at HML and HMR. This novel approach is independent of their switching function. HML is located on the left arm of chromosome III distal to his4 by about 26.8 centimorgans (cM). HMR maps on the right arm of the same chromosome distal to thr4 by about 39.8 cM and proximal to MAL2 by about 1.0 cM. The results allow the exact placement of these loci and are in accord with the observations made by Harashima and Oshima (1976).

Item Type: Paper
Uncontrolled Keywords: animal experiment fungus gene mapping heredity homothallism in vitro study saccharomyces cerevisiae Chromosome Mapping Crosses, Genetic Genes, Structural Histidine Leucine Phenotype Recombination, Genetic Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Threonine
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1980
Date Deposited: 09 Aug 2013 20:58
Last Modified: 12 Aug 2013 13:52
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28495

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