Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus

Strathern, J. N., Klar, A. J. S., Hicks, J. B., Abraham, J. A., Ivy, J. M., Nasmyth, K. A., McGill, C. (1982) Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus. Cell, 31 (1). pp. 183-192. ISSN 00928674 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/6297747

Abstract

A double-stranded DNA cut has been observed in the mating type (MAT) locus of the yeast Saccharomyces cerevisiae in cultures undergoing homothallic cassette switching. Cutting is observed in exponentially growing cells of genotype HO HMLα MATα HMRα or HO HMLa MATa HMRa, which switch continuously, but not in a/α HO/HO diploid strains, in which homothallic switching is known to be shut off. Stationary phase cultures do not exhibit the cut. Although this site-specific cut occurs in a sequence (Z1) common to the silent HML and HMR cassettes and to MAT, only the Z1 sequence at the MAT locus is cut. The cut at MAT occurs in the absence of the HML and HMR donor cassettes, suggesting that cutting initiates the switching process. An assay for switching on hybrid plasmids containing mata - cassettes has been devised, and deletion mapping has shown that the cut site is required for efficient switching. Thus a double-stranded cut at the MAT locus appears to initiate cassette transposition-substitution and defines MAT as the recipient in this process. © 1982.

Item Type: Paper
Uncontrolled Keywords: fungal DNA restriction endonuclease article chromosome chromosome map cross breeding diploidy genetics genotype nucleic acid hybridization plasmid Saccharomyces cerevisiae species difference Chromosome Mapping Chromosomes Crosses, Genetic DNA Restriction Enzymes DNA, Fungal Plasmids Species Specificity Support, U.S. Gov't, P.H.S.
Subjects: organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1982
Date Deposited: 09 Aug 2013 20:57
Last Modified: 12 Aug 2013 14:22
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28486

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