Translating Next Generation Sequencing to practice: opportunities and necessary steps

Kamalakaran, Sitharthan, Varadan, Vinay, Janevski, Angel, Banerjee, Nilanjana, Tuck, David, McCombie, Richard, Dimitrova, Nevenka, Harris, Lyndsay N. (2013) Translating Next Generation Sequencing to practice: opportunities and necessary steps. Molecular Oncology, 7 (4). pp. 743-755. ISSN 1574-7891

URL: http://www.ncbi.nlm.nih.gov/pubmed/23769412
DOI: 10.1016/j.molonc.2013.04.008

Abstract

Abstract Next-generation sequencing (NGS) approaches for measuring RNA and DNA benefit from greatly increased sensitivity, dynamic range and detection of novel transcripts. These technologies are rapidly becoming the standard for molecular assays and represent huge potential value to the practice of oncology. However, many challenges exist in the transition of these technologies from research application to clinical practice. This review discusses the value of NGS in detecting mutations, copy number changes and RNA quantification and their applications in oncology, the challenges for adoption and the relevant steps that are needed for translating this potential to routine practice.

Item Type: Paper
Uncontrolled Keywords: Next Generation Sequencing Oncology personalized medicine genomics
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
Investigative techniques and equipment > assays > next generation sequencing
bioinformatics > genomics and proteomics > personal genomes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes > personal genomes
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 22 May 2013 20:51
Last Modified: 16 Aug 2013 18:59
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28319

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