SEMA3A Signaling Controls Layer-Specific Interneuron Branching in the Cerebellum

Cioni, J. M., Telley, L., Saywell, V., Cadilhac, C., Jourdan, C., Huber, A.B., Huang, J. Z., Jahannault-Talignani, C., Ango, F. (2013) SEMA3A Signaling Controls Layer-Specific Interneuron Branching in the Cerebellum. Current Biology, 23 (10). pp. 850-861. ISSN 09609822

URL: http://www.ncbi.nlm.nih.gov/pubmed/23602477
DOI: 10.1016/j.cub.2013.04.007

Abstract

BACKGROUND:GABAergic interneurons regulate the balance and dynamics of neural circuits, in part, by elaborating their strategically placed axon branches that innervate specific cellular and subcellular targets. However, the molecular mechanisms that regulate target-directed GABAergic axon branching are not well understood. RESULTS:Here we show that the secreted axon guidance molecule, SEMA3A, expressed locally by Purkinje cells, regulates cerebellar basket cell axon branching through its cognate receptor Neuropilin-1 (NRP1). SEMA3A was specifically localized and enriched in the Purkinje cell layer (PCL). In sema3A-/- and nrp1sema-/sema- mice lacking SEMA3A-binding domains, basket axon branching in PCL was reduced. We demonstrate that SEMA3A-induced axon branching was dependent on local recruitment of soluble guanylyl cyclase (sGC) to the plasma membrane of basket cells, and sGC subcellular trafficking was regulated by the Src kinase FYN. In fyn-deficient mice, basket axon terminal branching was reduced in PCL, but not in the molecular layer. CONCLUSIONS:These results demonstrate a critical role of local SEMA3A signaling in layer-specific axonal branching, which contributes to target innervation.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > small molecules > GABA
organs, tissues, organelles, cell types and functions > tissues types and functions > cerebellum
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons

organs, tissues, organelles, cell types and functions > tissues types and functions > transport > membrane transport
CSHL Authors:
Communities: CSHL labs > Huang lab
Depositing User: Matt Covey
Date Deposited: 22 May 2013 20:22
Last Modified: 07 Apr 2015 15:56
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28317

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