Reprogramming the Epigenome in Arabidopsis Pollen

Borges, F., Calarco, J. P., Martienssen, R. A. (2013) Reprogramming the Epigenome in Arabidopsis Pollen. Cold Spring Harbor Symposia on Quantitative Biology, 77. pp. 1-5. ISSN 00917451 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/23619015
DOI: 10.1101/sqb.2013.77.014969

Abstract

Epigenetic reprogramming in Arabidopsis thaliana occurs in developing pollen. The male gametophyte is derived from haploid microspores via two postmeiotic cell divisions to give rise to the gametes (sperm cells, SC) and the vegetative cell (VC). The purification of individual cell types during pollen development coupled with genome-wide DNA methylation analysis and small RNA sequencing has revealed a dynamic regulation of the epigenome during gametogenesis. Interestingly, imprinted loci and previously identified variable epialleles are hypermethylated in the germline; however, their stability after fertilization appears to require targeted demethylation in the neighboring vegetative cell nucleus, possibly by releasing mobile small RNAs that reinforce transcriptional gene silencing and DNA methylation in the gametes. These results have led to a new model for the establishment and transgenerational maintenance of epigenetic marks in flowering plants.

Item Type: Paper
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > plant
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Watson School > Publications
Depositing User: Matt Covey
Date Deposited: 22 May 2013 20:07
Last Modified: 22 Sep 2014 20:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28315

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