A low-cost technique to cryo-protect and freeze rodent brains, precisely aligned to stereotaxic coordinates for whole-brain cryosectioning

Pinskiy, V., Tolpygo, A. S., Jones, J., Weber, K., Franciotti, N., Mitra, P. P. (2013) A low-cost technique to cryo-protect and freeze rodent brains, precisely aligned to stereotaxic coordinates for whole-brain cryosectioning. Journal of Neuroscience Methods, 218 (2). pp. 206-21. ISSN 0165-0270

URL: http://www.ncbi.nlm.nih.gov/pubmed/23541995
DOI: 10.1016/j.jneumeth.2013.03.004

Abstract

A major challenge in the histological sectioning of brain tissue is achieving accurate alignment in the standard coronal, horizontal, or sagittal planes. Correct alignment is desirable for ease of subsequent analysis and is a prerequisite for computational registration and algorithm-based quantification of experimental data. We have developed a simple and low-cost technique for whole-brain cryosectioning of rodent brains that reliably results in a precise alignment of stereotactic coordinates. The system utilises a 3-D printed model of a mouse brain to create a tailored cavity that is used to align and support the brain during freezing. The alignment of the frozen block is achieved in relation to the fixed edge of the mould. The system also allows for two brains to be frozen and sectioned simultaneously. System components, procedural steps, and examples of the end results are presented.

Item Type: Paper
Subjects: Investigative techniques and equipment
organs, tissues, organelles, cell types and functions > organs types and functions > brain
Investigative techniques and equipment > freezing
organs, tissues, organelles, cell types and functions > organs types and functions
organs, tissues, organelles, cell types and functions
organism description > animal > mammal > rodent
CSHL Authors:
Communities: CSHL labs > Mitra lab
Depositing User: Matt Covey
Date: 2013
Date Deposited: 22 May 2013 18:09
Last Modified: 03 Feb 2017 15:31
PMCID: PMC4265468
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28304

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