Molecular cloning and functional expression of a serotonin receptor from Caenorhabditis elegans

Olde, B., McCombie, W. R. (1997) Molecular cloning and functional expression of a serotonin receptor from Caenorhabditis elegans. Journal of Molecular Neuroscience, 8 (1). pp. 53-62. ISSN 08958696 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9061615
DOI: 10.1007/BF02736863

Abstract

A cDNA encoding a serotonin receptor has been isolated from a Caenorhabditis elegans mixed stage cDNA library. The nematode serotonin receptor, designated 5HT-Ce, was permanently expressed in murine Ltk- cells, where it mediates adenylate cyclase attenuation. Sequence analysis and the pharmacological profiles demonstrate its relatedness not only to Drosophila and Lymnae 5HT receptors but also to mammalian 5HT1a receptors. The 5HT-Ce gene does not map close to the position of any known serotonergic mutations. Copyright © 1997 Humana Press Inc. All rights of any nature whatsoever reserved.

Item Type: Paper
Uncontrolled Keywords: 5HT receptor Caenorhabditis elegans Cloning Expression adenylate cyclase complementary DNA serotonin serotonin receptor animal article cell strain COS1 chromosome map drug antagonism enzymology gene expression genetics metabolism molecular cloning molecular genetics mutation nucleotide sequence physiology sequence homology Animals Base Sequence Chromosome Mapping Cloning, Molecular COS Cells DNA, Complementary Molecular Sequence Data Receptors, Serotonin Sequence Homology, Amino Acid
Subjects: organism description > animal > C elegans
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > animal
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 23 Apr 2013 16:02
Last Modified: 23 Apr 2013 16:02
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28234

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