Genetic definition and sequence analysis of Arabidopsis centromeres

Copenhaver, G. P., Nickel, K., Kuromori, T., Benito, M. I., Kaul, S., Lin, X., Bevan, M., Murphy, G., Harris, B., Parnell, L. D., McCombie, W. R., Martienssen, R. A., Marra, M., Preuss, D. (1999) Genetic definition and sequence analysis of Arabidopsis centromeres. Science, 286 (5449). pp. 2468-2474. ISSN 00368075 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10617454
DOI: 10.1126/science.286.5449.2468

Abstract

High-precision genetic mapping was used to define the regions that contain centromere functions on each natural chromosome in Arabidopsis thaliana. These regions exhibited dramatic recombinational repression and contained complex DNA surrounding large arrays of 180-base pair repeats. Unexpectedly, the DNA within the centromeres was not merely structural but also encoded several expressed genes. The regions flanking the centromeres were densely populated by repetitive elements yet experienced normal levels of recombination. The genetically defined centromeres were well conserved among Arabidopsis ecotypes but displayed limited sequence homology between different chromosomes, excluding repetitive DNA. This investigation provides a platform for dissecting the role of individual sequences in centromeres in higher eukaryotes.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis article centromere gene mapping genetic analysis genetic recombination nonhuman priority journal sequence analysis Base Composition Base Sequence Conserved Sequence Contig Mapping Crosses, Genetic Crossing Over, Genetic DNA, Plant Gene Expression Genes, Plant Meiosis Models, Genetic Recombination, Genetic Repetitive Sequences, Nucleic Acid Retroelements Sequence Analysis, DNA Arabidopsis thaliana Embryophyta Eukaryota
Subjects: bioinformatics > genomics and proteomics > analysis and processing
organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
organism description > plant
CSHL Authors:
Communities: CSHL labs > Martienssen lab
CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 25 Apr 2013 15:53
Last Modified: 25 Apr 2013 15:53
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28215

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