Gene trap tagging of PROLIFERA, an essential MCM2-3-5-like gene in Arabidopsis

Springer, P. S., McCombie, W. R., Sundaresan, V., Martienssen, R. A. (1995) Gene trap tagging of PROLIFERA, an essential MCM2-3-5-like gene in Arabidopsis. Science, 268 (5212). pp. 877-880. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/7754372
DOI: 10.1126/science.7754372

Abstract

Gene trap transposon mutagenesis can identify essential genes whose functions in later development are obscured by an early lethal phenotype. In higher plants, many genes are required for haploid gametophyte viability, so that the phenotypic effects of their disruption cannot be readily observed in the diploid plant body. The PROLIFERA (PRL) gene, identified by gene trap transposon mutagenesis in Arabidopsis, is required for megagametophyte and embryo development. Reporter gene expression patterns revealed that PRL was expressed in dividing cells throughout the plant. PRL is related to the MCM2-3-5 family of yeast genes that are required for the initiation of DNA replication.

Item Type: Paper
Uncontrolled Keywords: mediated enhancer detection regulatory elements cell-cycle drosophila thaliana expression amplification meristem mutation excision
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Martienssen lab
CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 25 Apr 2013 15:51
Last Modified: 25 Apr 2013 15:51
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28214

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