Caenorhabditis elegans expressed sequence tags identify gene families and potential disease gene homologues

McCombie, W. R., Adams, M. D., Kelley, J. M., FitzGerald, M. G., Utterback, T. R., Khan, M., Dubnick, M., Kerlavage, A. R., Venter, J. C., Fields, C. (1992) Caenorhabditis elegans expressed sequence tags identify gene families and potential disease gene homologues. Nature Genetics, 1 (2). pp. 124-131. ISSN 10614036 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1302005
DOI: 10.1038/ng0592-124

Abstract

A database containing mapped partial cDNA sequences from Caenorhabdhitis elegans will provide a ready starting point for identifying nematode homologues of important human genes and determining their functions in C. elegans. A total of 720 expressed sequence tags (ESTs) have been generated from 585 clones randomly selected from a mixed-stage C. elegans cDNA library. Comparison of these ESTs with sequence databases identified 422 new C. elegans genes, of which 317 are not similar to any sequences in the database. Twenty-six new genes have been mapped by YAC clone hybridization. Members of several gene families, including cuticle collagens, GTP-binding proteins, and RNA helicases were discovered. Many of the new genes are similar to known or potential human disease genes, including CFTR and the LDL receptor.

Item Type: Paper
Uncontrolled Keywords: collagen DNA amino acid sequence animal article Caenorhabditis elegans gene gene expression gene library genetics human molecular cloning molecular genetics multigene family sequence homology species difference Cloning, Molecular Genes, Helminth Molecular Sequence Data Sequence Homology, Amino Acid Species Specificity
Subjects: organism description > animal > C elegans
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
diseases & disorders
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > animal
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 25 Apr 2013 20:36
Last Modified: 25 Apr 2013 20:36
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28197

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