Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3

Martin-Gallardo, A., McCombie, W. R., Gocayne, J. D., FitzGerald, M. G., Wallace, S., Lee, B. M. B., Lamerdin, J., Trapp, S., Kelley, J. M., Liu, L. I., Dubnick, M., Johnston-Dow, L. A., Kerlavage, A. R., De Jong, P., Carrano, A., Fields, C., Venter, J. C. (1992) Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3. Nature Genetics, 1 (1). pp. 34-39. ISSN 10614036 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1301997
DOI: 10.1038/ng0492-34

Abstract

A total of 116,118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.

Item Type: Paper
Uncontrolled Keywords: bacterial protein DNA fosB protein amino acid sequence animal article chromosome 19 comparative study cosmid DNA sequence gene expression genetics human methodology molecular genetics mouse nucleotide repeat nucleotide sequence oncogene sequence homology species difference Bacterial Proteins Base Sequence Chromosomes, Human, Pair 19 Cosmids Genes, fos Mice Molecular Sequence Data Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Sequence Homology, Nucleic Acid Species Specificity
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 25 Apr 2013 20:32
Last Modified: 25 Apr 2013 20:32
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28195

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